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Selective enrichment of polyunsaturated fatty acids by hydrolysis of fish oil using immobilized and stabilized Rhizomucor miehei lipase preparations

Paper ID Volume ID Publish Year Pages File Format Full-Text
18934 43037 2015 8 PDF Available
Title
Selective enrichment of polyunsaturated fatty acids by hydrolysis of fish oil using immobilized and stabilized Rhizomucor miehei lipase preparations
Abstract

•Immobilization of Rhizomucor miehei lipase on silica functionalized support was performed by using two different methods.•Immobilized derivative obtained by oriented immobilization had the best stability in presence of polar organic solvents.•The soluble enzyme and immobilized derivatives discriminate between EPA and DHA in favor of EPA.•The highest selectivity in fish oil hydrolysis reaction was observed by silica-epoxy-IDA-RML at pH 7 and 25 °C.•The immobilized derivatives showed high reusability in the hydrolysis reaction.

Immobilization of Rhizomucor miehei lipase (RML) by two different methods is reported. In the first approach RML was directly immobilized on epoxy functionalized silica particles in a mild condition. In the other approach and to perform oriented immobilization, the epoxy functionalized support was partially modified by introducing iminodiacetic acid groups fallowed by addition of Cu2+. In this type of immobilization the covalent linkage takes place after initial adsorption of the enzyme on the support via metal chelate affinity interaction. The results showed higher thermal stability for the derivative obtained by this method. Co-solvent stability of the derivatives and free RML was also studied in presence of 10 and 20% of six polar organic solvents (DMSO, THF, acetonitrile, 1-propanol, 2-propanol and dioxane). The results showed improved stabilities for both derivatives, silica-epoxy-IDA-RML in particular. The ability of the immobilized preparations and the free enzyme to catalyze hydrolysis of fish oil was determined at different conditions. The results revealed that all the derivatives discriminate between cis-5,8,11,14,17-eicosapentaenoic acid (EPA) and cis-4,7,10,13,16,19-docosahexaenoic acid (DHA) in favor of EPA. The content of EPA and DHA increased with time and the amount of biocatalyst during the hydrolysis process. Reusability of the enzyme greatly improved after immobilization.

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Keywords
Rhizomucor miehei lipase; Oriented immobilization; Silica; Selective hydrolysis; Fish oil; Polyunsaturated fatty acids
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Selective enrichment of polyunsaturated fatty acids by hydrolysis of fish oil using immobilized and stabilized Rhizomucor miehei lipase preparations
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Publisher
Database: Elsevier - ScienceDirect
Journal: Food and Bioproducts Processing - Volume 94, April 2015, Pages 414–421
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us