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New proteases extracted from red scorpionfish (Scorpaena scrofa) viscera: Characterization and application as a detergent additive and for shrimp waste deproteinization

Paper ID Volume ID Publish Year Pages File Format Full-Text
18938 43037 2015 10 PDF Available
Title
New proteases extracted from red scorpionfish (Scorpaena scrofa) viscera: Characterization and application as a detergent additive and for shrimp waste deproteinization
Abstract

•Alkaline proteases from S. scrofa viscera were extracted and characterized.•The optimum pH and temperature were 10.0 and 55 ̊C, respectively.•The crude enzyme was extremely stable in alkaline conditions and towards detergents.•The crude enzyme was highly recommended for application in detergent formulations.•The crude enzyme was also recommended for shrimp shell deproteinization.

Alkaline proteases from the red scorpionfish (Scorpaena scrofa) viscera were extracted and characterized. This proteolytic crude extract was active and stable in alkaline solution: it was extremely stable in the pH range of 5.0–12.0; the optimum pH and temperature were 10.0 and 55 °C, respectively, using casein as a substrate. Three caseinolytic proteases clear bands were observed in the zymogram test suggesting the presence of at least three different major proteinases.This proteolytic crude extract was then tested for its potential applications, especially in laundry detergents and shrimp waste treatment. Results showed that it was extremely stable toward non-ionic surfactants (Tween 20, Tween 80 and Triton X-100). It was also stable in the presence of oxidizing agents, retaining 100% of its initial activity in the presence of 1% sodium perborate. Interestingly, it showed relative stability and compatibility with commercial liquid and solid detergents. These properties and the high activity in high alkaline pH make these proteases an ideal choice for application in detergent formulations.Additionally, S. scrofa proteases were found to be effective in the deproteinization of shrimp waste. A high level of deproteinization 83 ± 1.3% was recorded with an E/S ratio of 10 U/mg after 3 h incubation at 50 °C. These results suggest that enzymatic deproteinization of the shrimp waste, using S. scrofa proteases, could be applicable to the chitin production process.

Keywords
Alkaline proteases; Scorpaena scrofa; Enzymatic deproteinization; Shrimp waste; Chitin; Detergent additive
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New proteases extracted from red scorpionfish (Scorpaena scrofa) viscera: Characterization and application as a detergent additive and for shrimp waste deproteinization
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Publisher
Database: Elsevier - ScienceDirect
Journal: Food and Bioproducts Processing - Volume 94, April 2015, Pages 453–462
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us