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Optimization and evaluation of a novel technique for hydrolyzing Antarctic krill (Euphausia superba) proteins

Paper ID Volume ID Publish Year Pages File Format Full-Text
18958 43037 2015 8 PDF Available
Title
Optimization and evaluation of a novel technique for hydrolyzing Antarctic krill (Euphausia superba) proteins
Abstract

The endogenous proteases of Antarctic krill (Euphausia superba) were efficiently utilized to hydrolyze proteins by a novel technique called two-stage autolysis. The hydrolysates contained 0.951 g/100 ml amino acid nitrogen, which was sufficient to meet the special grade requirements of soy sauce according to the Chinese National Standard. Meanwhile, the recovery of total nitrogen was 75.33%. The second-stage autolysis exhibited a great positive effect on the improvement of nitrogen recovery. The optimal conditions for obtaining the highest amino acid nitrogen level during the second-stage autolysis were hydrolysate/krill ratio of 2.23, initial pH of 7.23, 40.94 °C, which were determined by response surface methodology. The dominant compositions of krill protein hydrolysates were peptides. Proline, glutamate, aspartate, glycine and valine were the dominant amino acids in the hydrolysates, accounting for 15.18, 14.03, 12.14, 10.78 and 10.16 mg/ml, respectively. The hydrolysates prepared by two-stage autolysis had high amino acid nitrogen level and high recovery of total nitrogen, which could be used in the preparation of high quality krill sauce.

Keywords
Euphausia superba; Antarctic krill; Endogenous protease; Autolysis; Hydrolysate; Amino acid nitrogen; Total nitrogen; Response surface methodology
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Optimization and evaluation of a novel technique for hydrolyzing Antarctic krill (Euphausia superba) proteins
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Publisher
Database: Elsevier - ScienceDirect
Journal: Food and Bioproducts Processing - Volume 94, April 2015, Pages 629–636
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us