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Extraction, purification and application of thermostable and halostable alkaline protease from Bacillus alveayuensis CAS 5 using marine wastes

Paper ID Volume ID Publish Year Pages File Format Full-Text
18969 43038 2014 8 PDF Available
Title
Extraction, purification and application of thermostable and halostable alkaline protease from Bacillus alveayuensis CAS 5 using marine wastes
Abstract

•Study proposed on utilization of marine waste for inexpensive protease production.•The protease from this study had higher temperature, pH and detergents stability.•Application in stain removal could be useful in textile industries.•Considering production cost and reclamation of waste seems to be promising approach.

A marine bacterium Bacillus alveayuensis CAS 5 produced protease when grown at 55 °C for 60 h in 100 ml of basal medium containing 1% SSP (w/v) and purified to 7.77 fold with specific activity of 518.78 U/mg. The optimum temperature, pH and NaCl for enzyme activity were 50 °C, 9 and 35% respectively. The enzyme was highly stable even at 80 °C, pH 12, 35% NaCl and presence of ionic, non-ionic and commercial detergents. The protease was investigated for its application as cleansing additive in blood stain removal. The preset study emphasized that marine wastes can be utilized to generate high value-added products and hidden potential in the production of functional foods.

Keywords
Bacillus alveayuensis; Marine wastes; Protease; Utilization; Thermostable; Antioxidant
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Extraction, purification and application of thermostable and halostable alkaline protease from Bacillus alveayuensis CAS 5 using marine wastes
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Publisher
Database: Elsevier - ScienceDirect
Journal: Food and Bioproducts Processing - Volume 92, Issue 4, October 2014, Pages 335–342
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us