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Development of a molecularly imprinted polymer for the recovery of lactoferrin

Paper ID Volume ID Publish Year Pages File Format Full-Text
18993 43040 2014 7 PDF Available
Title
Development of a molecularly imprinted polymer for the recovery of lactoferrin
Abstract

•Molecular imprinting was successfully used to separate a valuable protein from a complex mixture.•Accessible Surface Area proved to be a valuable tool for the selection of proper functional monomer.•Molecular imprinting could be us for the purification of valuable whey proteins.

Lactoferrin (LF) was recovered by molecular imprinting using either vinylpirydin alone (MIP1) or mixed with methacrylic acid (MIP2) as functional monomers. In order to create the specific cavity, pure LF was used as template. Controls were prepared for every MIP. The polymers obtained were tested against a protein mixture containing LF, and the recovery efficiency was calculated determining the diminution of LF from the supernatant by titration of the iron of the LF, as well as native PAGE to determine the protein composition of the supernatants. The only polymer which was able to specifically bind LF was MIP1 (vinylpirydin alone). Measuring the proportions of the different proteins in the mixture, it was demonstrated that before the separation with MIP1, LF represented 81.9% of the total protein content of the mixture, and diminished to 70.5% after the exposure to MIP1, suggesting that LF was specifically sorbed by this polymer. The retention efficiency of the polymer showed that MIP1 retained 34.5% of the total LF content, while its control did not retain any, demonstrating that the retention of the protein is not due to unspecific adsorption in the polymer, but rather to a selective retention in the cavity formed by the template.

Keywords
Lactoferrin; Whey proteins; Molecular imprinting; Solvent-Accessible Surface analysis
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Development of a molecularly imprinted polymer for the recovery of lactoferrin
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Publisher
Database: Elsevier - ScienceDirect
Journal: Food and Bioproducts Processing - Volume 92, Issue 2, April 2014, Pages 226–232
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
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Any Questions? feel free to contact us