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Microbiological efficacy of pressure assisted thermal processing and natural extracts against Bacillus amyloliquefaciens spores suspended in deionized water and beef broth

Paper ID Volume ID Publish Year Pages File Format Full-Text
19016 43041 2015 9 PDF Available
Title
Microbiological efficacy of pressure assisted thermal processing and natural extracts against Bacillus amyloliquefaciens spores suspended in deionized water and beef broth
Abstract

•Natural extracts enhanced PATP inactivation of B. amyloliquefaciens spores.•Natural extracts showed an additive effect with PATP.•PATP resulted in 4.5 to 6.5 log CFU ml−1 inactivation of B. amyloliquefaciens spores.•Inactivation of B. amyloliquefaciens spores by HPP and TP was 0.5–1 log CFU ml−1.

Effect of aqueous extracts of pomegranate peel (PPE) and tamarind pulp (TPE) on lethality of pressure assisted thermal processing (PATP) against Bacillus amyloliquefaciens Fad 82 spores was investigated. B. amyloliquefaciens spores (≈108 CFU ml−1) were suspended in deionized water (DIW), 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer PPE, and TPE (1% w/v in DIW, pH-6) and beef broth with or without PPE and TPE (0.45% w/v). Samples were subjected to pressure assisted thermal processing (PATP, 600 MPa, 105 °C), and thermal processing (TP; 0.1 MPa, 105 °C) for 0 (come-up time), 0.5, 1, 3, and 5 min. Beef broth samples were also pressure treated (HPP; 600 MPa, 35 °C) for 3 and 5 min. Dormant spore survivors were enumerated by spread-plate technique. Heat shock (80 °C for 15 min) was used to enumerate the heat sensitive spores. A 5-min PATP reduced spore survivors counts to below 1-log CFU ml−1 (≈6.5–7.0 log reduction) in PPE and TPE as compared to 2.5-log and 3.1-log in DIW and HEPES buffer, respectively. 3 min PATP treatment reduced the spore survival to 3.58 and 2.1 log CFU ml−1 in beef broth and beef broth + natural extract, respectively. TP and HPP processing could inactivate the spores only by 0.5–1.2 log CFU ml−1. PPE and TPE are found to enhance the efficacy of PATP.

Keywords
Pressure assisted-thermal processing; High pressure processing; Natural extracts; Spore inactivation; Beef broth; Natural antimicrobials
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Microbiological efficacy of pressure assisted thermal processing and natural extracts against Bacillus amyloliquefaciens spores suspended in deionized water and beef broth
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Publisher
Database: Elsevier - ScienceDirect
Journal: Food and Bioproducts Processing - Volume 95, July 2015, Pages 183–191
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us