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Enzymolysis kinetics of garlic powder with single frequency countercurrent ultrasound pretreatment

Paper ID Volume ID Publish Year Pages File Format Full-Text
19027 43041 2015 6 PDF Available
Title
Enzymolysis kinetics of garlic powder with single frequency countercurrent ultrasound pretreatment
Abstract

•Effect of ultrasound on kinetic constants of garlic enzymolysis was investigated.•Hydrolysis curves of garlic catalyzed by alcalase were determined.•The enzymolysis process was modeled over a wide range of operating conditions.

This research was to describe the enzymolysis process of garlic powder which was pretreated by single frequency countercurrent ultrasound. The kinetic constants for ultrasonic pretreated and traditional enzymolysis have been determined. Results showed the value of KM in ultrasonic pretreated enzymolysis decreased by 12.7% over the traditional enzymolysis, which indicates a fast reaction speed induced by ultrasound pretreatment. Degrees of hydrolysis (DH) in the enzymolysis of garlic powder were determined and kinetics of the reaction was considered in detail in relation to substrate concentration, enzyme concentration, reaction time and DH. Our results suggested a general kinetic equation for the enzymolysis model of ultrasonic pretreated garlic powder. For the model system, the calculated values agree well with the experimental data with average relative error of 4.42%. The proposed kinetic equation can be used to model the bio-reaction process of protein enzymatic hydrolysis and optimize the operation parameters for bioreactor design.

Keywords
Ultrasound; Enzymolysis; Kinetics; Kinetic constants; Garlic; Enzymolysis model
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Publisher
Database: Elsevier - ScienceDirect
Journal: Food and Bioproducts Processing - Volume 95, July 2015, Pages 292–297
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us