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Nanodiamonds as an effective and novel matrix for immobilizing β galactosidase

Paper ID Volume ID Publish Year Pages File Format Full-Text
19028 43041 2015 6 PDF Available
Title
Nanodiamonds as an effective and novel matrix for immobilizing β galactosidase
Abstract

•Nanodiamonds provided large surface area for immobilization of β-galactosidase.•Immobilized enzyme showed greater biocatalytic activity in terms of selectivity, loading and stability.•Immobilized β galactosidase exhibited greater hydrolysis of lactose from solution as compared to soluble enzyme.

The present study demonstrates the biospecific adsorption of Aspergillus oryzae β galactosidase on glutaraldehyde functionalized nanodiamonds (NDs). Transmission electron microscopy showed that the synthesized NDs were of 20 nm size. Enzyme activity retained as a result of immobilization was 7420 U/gm of modified NDs. The optimal pH and temperature for soluble and immobilized β galactosidase was observed at pH 4.5 and at 50 °C, respectively. However, significant stability was observed at both higher and lower limits of pH and temperature for the enzyme immobilized on glutaraldehyde modified NDs. Moreover, our findings demonstrated that β galactosidase immobilized on surface functionalized NDs retained greater biocatalytic activity even after 2 months of storage and at higher galactose concentration, and upon repeated uses as compared to enzyme in solution. Modified NDs bound β galactosidase showed improved hydrolysis of lactose from solution in batch processes at various temperatures even after 10 h, thereby suggesting its use for hydrolyzing lactose in dairy products.

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Keywords
NDs, nanodiamonds; ONPG, o-nitrophenyl β-d-galctopyranoside; SβG, soluble β galactosidase; IβG, immobilized β galactosidase (enzyme immobilized on glutaraldehyde modified NDs)β Galactosidase; Nanodiamonds; Glutaraldehyde; Lactose hydrolysis; Stability; Da
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Nanodiamonds as an effective and novel matrix for immobilizing β galactosidase
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Publisher
Database: Elsevier - ScienceDirect
Journal: Food and Bioproducts Processing - Volume 95, July 2015, Pages 298–303
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us