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An alternative method for the decoloration of ɛ-poly-l-lysine eluate by macroporous resin in the separation and purification of ɛ-poly-l-lysine from fermentation broth

Paper ID Volume ID Publish Year Pages File Format Full-Text
19032 43041 2015 7 PDF Available
Title
An alternative method for the decoloration of ɛ-poly-l-lysine eluate by macroporous resin in the separation and purification of ɛ-poly-l-lysine from fermentation broth
Abstract

•Macroporous resin was firstly proposed to the decoloration of the ɛ-PL eluate.•The conditions of the decoloration by SX-8 resin were optimized.•SX-8 resin carried out the high decoloration and ɛ-PL recovery ratios.•Pigments with absorption at 300–900 nm were effectively removed.•This method is promising for the scale-up production of ɛ-PL.

To develop an efficient strategy for the decoloration of ɛ-poly-l-lysine (ɛ-PL) produced by microbial fermentation with Streptomyces, macroporous resin was firstly investigated on the removal of pigments in ɛ-PL ion exchange eluate here. SX-8 resin was found to be the best through static and dynamic comparison tests, which adsorbed the most pigments and the least ɛ-PL. Under the optimized dynamic adsorption conditions, i.e. pH 7.0, 30° C, 1 BV/h uploading rate, 7 g/L ɛ-PL concentration, SX-8 resin was employed to the treatment of 100 mL (10 BV) ɛ-PL ion exchange eluate, and the resulting decoloration ratio was 83.9% with a recovery ratio of ɛ-PL 97.3%. Further spectrum analysis showed that pigments with absorption wavelength between 300 and 900 nm were effectively removed after treatment by SX-8 resin. The results presented reveal that SX-8 resin is promising for scale-up ɛ-PL extraction and purification from fermentation broth.

Keywords
Macroporous resin; ɛ-Poly-l-lysine; Biological food preservative; Fermentation broth; Decoloration; Adsorption
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An alternative method for the decoloration of ɛ-poly-l-lysine eluate by macroporous resin in the separation and purification of ɛ-poly-l-lysine from fermentation broth
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Publisher
Database: Elsevier - ScienceDirect
Journal: Food and Bioproducts Processing - Volume 95, July 2015, Pages 332–338
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us