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Pineapple wastes: A potential source for bromelain extraction

Paper ID Volume ID Publish Year Pages File Format Full-Text
19052 43043 2012 7 PDF Available
Title
Pineapple wastes: A potential source for bromelain extraction
Abstract

This study investigates the isolation and characterization of bromelain extract from the wastes of Nang Lae and Phu Lae pineapple cultivars (economical fruits of Chiang Rai province, Thailand). The waste portions such as the peel, core, stem and crown were 29–40%, 9–10%, 2–5% and 2–4% (w/w), respectively. The extract of crown from both cultivars gave the highest proteolytic activity and protein contents, while the extract from the stem exhibited the lowest values. SDS–PAGE showed that the major protein band in the extracts was ∼28 kDa. Activity staining of the crown extracts from both cultivars confirmed that the major protein band showed caseinolytic activity on the casein substrate-gel. All of the crude extracts from both cultivars gave high caseinolytic activity (>80% relative) in a board pH range (3–9). The optimum temperatures for all crude extracts were about 50–60 °C. This study founded that there is much added value into local Thailand pineapple wastes because of bromelain extraction.

► Pineapple wastes (peel, core, stem and crown) from 2 cultivars were used as materials for “bromelain” extraction. ► The peel gave the highest waste portion of the pineapples compared with others. ► Crown provided the highest protease activity in both cultivars followed by peel, cores, and stem, respectively.

Keywords
Bromelain; Extraction; Nang Lae; Phu Lae; Pineapple; Waste utilization
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Publisher
Database: Elsevier - ScienceDirect
Journal: Food and Bioproducts Processing - Volume 90, Issue 3, July 2012, Pages 385–391
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us