fulltext.study @t Gmail

Modeling the recovery patterns from solid phase extraction purification of secoisolariciresinol diglucoside, p-coumaric acid glucoside, and ferulic acid glucoside from microwave-assisted flaxseed extracts

Paper ID Volume ID Publish Year Pages File Format Full-Text
19061 43043 2012 13 PDF Available
Title
Modeling the recovery patterns from solid phase extraction purification of secoisolariciresinol diglucoside, p-coumaric acid glucoside, and ferulic acid glucoside from microwave-assisted flaxseed extracts
Abstract

Flaxseed extracts obtained by microwave-assisted extraction (MAE) were cleaned of leftover salts from hydrolysis by solid phase extraction (SPE). The SPE set up was affordable, non-automated and vacuum-driven. The recovery patterns of secoisolariciresinol diglucoside (SDG), p-coumaric acid glucoside (PCouAG), and ferulic acid glucoside (FerAG) were modeled in two stages using regression procedures (p < 0.05). At stage one, the recovery patterns were predicted as a function of SPE eluent concentration in ethanol (10–50%). At stage two, the accuracy of the predictions was increased by enlarging the SPE eluent regressor's range (0–100% ethanol in water) and arranging the solvent system into three practical elution groups. The groups 1, 2 and 3 reflected the major loss, the major recovery and the minor loss of SDG, respectively. Second degree polynomial regression models were fitted for accurately predicting the recoveries of compounds. Microwave-assisted extracts obtained from 0.6 and 1.5 g defatted flaxseed meal were purified; the total SDG recoveries from the SPE funnel were 97.8 and 99.8%; and the SDG amounts obtained were 8.54 and 20 mg, respectively. The HPLC analysis of eluates pooled into practical groups allows for significant reductions in HPLC analysis time and solvent consumption which could have a positive impact on future purification studies. The results of this study allow for designing simplified, efficient and economical pilot scale studies for the purification of SDG from flaxseed extracts.

► A SPE technique was developed for purification of flaxseed lignans. ► The purified compound recovery patterns were established using regression analysis. ► Using practical SPE elution groups as regression factors improves the predictions. ► The SPE groups reflect the loss, the major, and the minor recoveries of compounds. ► The concentration of extracts purified by SPE affect the compound elution profiles.

Keywords
Prediction accuracy; SDG; SPE; Purification losses; Lignan; MAE
First Page Preview
Modeling the recovery patterns from solid phase extraction purification of secoisolariciresinol diglucoside, p-coumaric acid glucoside, and ferulic acid glucoside from microwave-assisted flaxseed extracts
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Food and Bioproducts Processing - Volume 90, Issue 3, July 2012, Pages 453–465
Authors
, ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us