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Optimization of extraction and purification process of hyaluronic acid from fish eyeball

Paper ID Volume ID Publish Year Pages File Format Full-Text
19064 43043 2012 8 PDF Available
Title
Optimization of extraction and purification process of hyaluronic acid from fish eyeball
Abstract

The goal of the present work is to optimize the different steps for obtaining highly purified hyaluronic acid (HA) from fish eyeball. The extraction and purification process of HA from vitreous humour of fish, among other biological materials, is based on the succession of: (1) a step of protein electrodeposition, previous or simultaneous with a diafiltration process in total recirculation, (2) a selective recovery in hydroalcoholic solution of impure sediments obtained by alcoholic exhaustive precipitation, (3) an alkaline treatment under hydroalcoholic solution and controlled conditions of alkalinity, temperature, proportion of ethanol and time that it precipitates HA and solubilises proteins, and (4) HA recovery by alkaline suspension of the precipitate in hydroalcoholic phosphate monosodium that it dissolves HA, neutralizes the extract and leaves insoluble proteins in the sediment. Thus, HA with high purity (more than 99.5%), useful for clinical and cosmetic applications, are obtained by means of low-cost process using a waste material.

► The extraction and purification process of HA from vitreous humour of fish has been development. ► Different steps of protein electrodeposition, exhaustive precipitation, selective recovery and membrane separation were used. ► Optimization of all process was also carried out. ► HA with high purity was obtained by means of low-cost process using a waste material.

Keywords
Hyaluronic acid; Fish by-products; Bioprocessing; Environment; Food processing; Downstream processing
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Optimization of extraction and purification process of hyaluronic acid from fish eyeball
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Publisher
Database: Elsevier - ScienceDirect
Journal: Food and Bioproducts Processing - Volume 90, Issue 3, July 2012, Pages 491–498
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us