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Bulk preparation of (−)-epigallocatechin gallate-rich extract from green tea

Paper ID Volume ID Publish Year Pages File Format Full-Text
19083 43044 2014 7 PDF Available
Title
Bulk preparation of (−)-epigallocatechin gallate-rich extract from green tea
Abstract

•A three-stage process was developed for bulk preparation of EGCg-rich extract.•Either of crude tea extract or green tea leaf is viable as raw material.•High EGCg >700 mg g−1 and low caffeine <1 mg g−1 products are obtained.•Solvent extraction and silica column purification are the major procedures.•No toxic solvents or metal ions are used.

(−)-Epigallocatechin gallate (EGCg)-rich extract (EGCg > 700 mg g−1) was prepared from green tea leaves through a three-stage process consisting of liquid–liquid extraction and silica column purification. Crude tea extract was dissolved in ethyl acetate. After filtration, the solution was extracted by 10 g L−1 citric acid solution twice, and then passed through silica column. The catechins compounds in the ethyl acetate eluate were back extracted to the aqueous phase, then extracted with a mixed solution of n-hexane/ethyl acetate (2/5, v/v) 3 times, concentrated, and freeze dried. 12.8 g EGCg-rich extract containing 709 mg g−1 EGCg and 965 mg g−1 total catechins was obtained from 300 g green tea leaves, with an EGCg recovery of 26.1% and a yield of 4.3%. This method was suitable for bulk preparation of EGCg-rich catechins from green tea leaves.

Keywords
Catechins; EGCg; Liquid–liquid extraction; Silica column; Back extraction; Orthogonal study
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Bulk preparation of (−)-epigallocatechin gallate-rich extract from green tea
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Publisher
Database: Elsevier - ScienceDirect
Journal: Food and Bioproducts Processing - Volume 92, Issue 3, July 2014, Pages 275–281
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us