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Optimization of extracellular lipase production by Debaryomyces hansenii isolates from dry-salted olives using response surface methodology

Paper ID Volume ID Publish Year Pages File Format Full-Text
19107 43045 2013 8 PDF Available
Title
Optimization of extracellular lipase production by Debaryomyces hansenii isolates from dry-salted olives using response surface methodology
Abstract

The lipase production by Debaryomyces hansenii strains isolated from dry-salted olives cv. Thassos was investigated. Glucose, olive oil and pH were essential to obtain a high lipase yield. Optimization of the medium components which enhance lipase production by the strain D. hansenii YLL29 was achieved with the aid of response surface methodology. The composition of the optimized medium to enhance lipase production by D. hansenii is as follows (g/L): yeast extract 5.0, peptone 10, K2HPO4 4.0, MgSO4·7H2O 1.0, glucose 13.1, olive oil 19, Tween 80 3.8, and pH 6.4. Practical validation of the optimum medium gave lipase activity 7.44 U/mL, which was 2.28-fold higher than the unoptimized conditions. Under the optimized conditions the twenty D. hansenii isolates showed increased lipase activity fluctuating between 6.00 and 7.44 U/mL. The results corroborated the validity and the effectiveness of the model, as the statistical approaches proved to be suitable in predicting the optimum production medium composition for maximum extracellular lipase yield. The high lipolytic activity of D. hansenii YLL29 (7.44 U/mL) indicates the possible commercial importance of this isolate.

Graphical abstractFigure optionsDownload full-size imageDownload high-quality image (435 K)Download as PowerPoint slideHighlights► Debaryomyces hansenii strains were isolated from dry-salted olives. ► Central composite design was employed to optimize glucose, olive oil and pH for maximum lipase production. ► Increased lipase production by Debaryomyces hansenii isolates is growth associated.

Keywords
Extracellular lipase production; Debaryomyces hansenii; Batch culture; Dry-salted olives; Experimental design
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Optimization of extracellular lipase production by Debaryomyces hansenii isolates from dry-salted olives using response surface methodology
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Publisher
Database: Elsevier - ScienceDirect
Journal: Food and Bioproducts Processing - Volume 91, Issue 4, October 2013, Pages 413–420
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us