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Effect of cross-linking method on the activity of spray-dried chitosan microparticles with immobilized laccase

Paper ID Volume ID Publish Year Pages File Format Full-Text
19119 43045 2013 9 PDF Available
Title
Effect of cross-linking method on the activity of spray-dried chitosan microparticles with immobilized laccase
Abstract

•Enzyme immobilization method by spray drying with in situ cross-linking was developed.•Morphology and microstructure of chitosan microparticles were investigated.•Laccase was successfully immobilized and its activity was assessed.•Particles produced by in situ cross-linking have superior properties.

The effect of processing method on the properties of cross-linked chitosan microparticles and on the enzymatic activity of laccase immobilized in the particles has been investigated. Chitosan has been cross-linked by tri-polyphosphate (TPP) using two methods – the so called ex situ cross-linking whereby the solutions of chitosan, TPP and the enzyme have been pre-mixed and spray-dried by a standard two-fluid kinetic nozzle, and a novel in situ cross-linking method, whereby the solutions have been contacted at the tip of a three-fluid nozzle and cross-linking occurred within a drying droplet. The influence of the cross-linking method on the particle size and morphology, surface charge, and swelling ratio has been determined. The enzymatic activity of laccase toward the oxidation of a chromophore substrate (ABTS) has been systematically investigated and found to be superior in particles produced by the in situ cross-linking method.

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Keywords
Spray drying; Encapsulation; Enzyme; Kinetics; Swelling; Diffusion
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Effect of cross-linking method on the activity of spray-dried chitosan microparticles with immobilized laccase
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Publisher
Database: Elsevier - ScienceDirect
Journal: Food and Bioproducts Processing - Volume 91, Issue 4, October 2013, Pages 525–533
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
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Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
Online Support
Any Questions? feel free to contact us