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Fungal fucoidanase production by solid-state fermentation in a rotating drum bioreactor using algal biomass as substrate

Paper ID Volume ID Publish Year Pages File Format Full-Text
19127 43045 2013 8 PDF Available
Title
Fungal fucoidanase production by solid-state fermentation in a rotating drum bioreactor using algal biomass as substrate
Abstract

Fucoidanase enzymes able to degrade fucoidan were produced by solid-state fermentation (SSF). The fermentation assays were initially carried out in a laboratory-scale rotating drum bioreactor, and two fungal strains (Aspergillus niger PSH and Mucor sp. 3P) and three algal substrates (untreated, autohydrolyzed, and microwave processed seaweed Fucus vesiculosus) were evaluated. Additionally, fermentations were carried out under rotational (10 rpm) and static conditions in order to determine the effect of the agitation on the enzyme production. Agitated experiments showed advantages in the induction of the enzyme when compared to the static ones. The conditions that promoted the maximum fucoidanase activity (3.82 U L−1) consisted in using Mucor sp. 3P as fungal strain, autohydrolyzed alga as substrate, and the rotational system. Such conditions were subsequently used in a 10 times larger scale rotating drum bioreactor. In this step, the effect of controlling the substrate moisture during the enzyme production by SSF was investigated. Moreover, assays combining the algal substrate with an inert support (synthetic fiber) were also carried out. Fermentation of the autohydrolyzed alga with the moisture content maintained at 80% during the fermentation with Mucor sp. 3P gave the highest enzyme activity (9.62 U L−1).

Graphical abstractFigure optionsDownload full-size imageDownload high-quality image (185 K)Download as PowerPoint slideHighlights► Fucoidanases were produced by solid-state fermentation in a rotating drum bioreactor. ► Two fungal strains and three algal substrates were evaluated. ► Agitated experiments were better than the static ones for the enzyme production. ► The assays revealed the importance of controlling the moisture of the solid substrate. ► The enzyme production was maximized in the scale-up experiments (9.62 U L−1).

Keywords
SSF, solid-state fermentation; UA, untreated alga; AA, autohydrolyzed alga; MA, microwave processed alga; NAA, non-isothermal autohydrolyzed alga; SAA, non-isothermal autohydrolyzed alga supported on synthetic fiber cubesFilamentous fungi; Fucoidanase; Fu
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Fungal fucoidanase production by solid-state fermentation in a rotating drum bioreactor using algal biomass as substrate
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Publisher
Database: Elsevier - ScienceDirect
Journal: Food and Bioproducts Processing - Volume 91, Issue 4, October 2013, Pages 587–594
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us