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Papain from papaya (Carica papaya L.) fruit and latex: Preliminary characterization in alcoholic–acidic buffer for wine application

Paper ID Volume ID Publish Year Pages File Format Full-Text
19128 43045 2013 4 PDF Available
Title
Papain from papaya (Carica papaya L.) fruit and latex: Preliminary characterization in alcoholic–acidic buffer for wine application
Abstract

Papain from ripe fruit and from papaya latex was characterised, in comparison with stem bromelain, under wine-like conditions, with the aim to evaluate their applicability for white wine stabilization.Papains proteolytic activity was investigated, in McIlvaine buffer toward different synthetic peptide substrates and Bz-Phe-Val-Arg-pNA appeared the most suitable one for detecting proteolytic effect at wine average minimum pH (3.2). Kinetic parameters estimated in McIlvaine (as reference) and in tartaric buffer (mimicking wine medium), indicated a good hydrolytic activity toward selected substrate, at wine average minimum pH value (3.2) in spite of ethanol presence, in both mediums. Papain from latex showed a significantly higher and stable catalytic activity respect to fruit papain and stem bromelain, retaining after 7 days, about 50% of its initial activity.

► Papain showed a good hydrolytic activity toward Bz-Phe-Val-Arg-pNA substrate. ► Papain resulted active at wine average minimum pH value (3.2). ► Latex papain had significantly higher and stable activity respect to fruit papain.

Keywords
Fruit papain; Latex papain; Model wine; Synthetic peptide substrates; Catalytic activity; Stability
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Papain from papaya (Carica papaya L.) fruit and latex: Preliminary characterization in alcoholic–acidic buffer for wine application
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Publisher
Database: Elsevier - ScienceDirect
Journal: Food and Bioproducts Processing - Volume 91, Issue 4, October 2013, Pages 595–598
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us