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An efficient method for the enrichment of the arachidonic acid methyl ester from Mortierella alpina-derived crude oils

Paper ID Volume ID Publish Year Pages File Format Full-Text
19131 43045 2013 6 PDF Available
Title
An efficient method for the enrichment of the arachidonic acid methyl ester from Mortierella alpina-derived crude oils
Abstract

•We describe a simple separation method assisted solvents for the enrichment of arachidonic acid methyl ester.•Three methyl esterification methods were used to process the microbial oil derived from Mortierella alpina.•Arachidonic acid methyl ester obtained better enrichment efficiency and recovery.•This method simplifies the existing separation methods.

An improved method was developed for enriching arachidonic acid (AA) methyl ester from microbial oil by two-step low-temperature wet fractionation. The effects of solvent, operating temperature, and solvent-to-fatty acid methyl esters (FAMEs) ratio on the enrichment of AA were investigated. The best results were achieved when n-hexane was used as solvent. With operating temperatures in the range −30 °C to −80 °C and a FAMEs-to-solvent ratio of 1:5 (v/v), the proportion of AA methyl ester isolated could be increased to 83.76 ± 2.78% with a yield of 52.89%. The total recovery of AA methyl ester would be further increased to 90.84% by recrystallization of the solid phases. The 20C, 22C saturated fatty acids were enriched by n-hexane or petroleum ether at −30 °C, with concentrations increased 7.5-fold or 7.2-fold compared with their original levels, respectively. In addition, a method that combined alkali and acid catalysis of the transmethylation was the most conducive to the preparation of polyunsaturated fatty acid methyl esters.

Keywords
Arachidonic acid; n-Hexane; Microbial oil; Two-step low temperature fractionation
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An efficient method for the enrichment of the arachidonic acid methyl ester from Mortierella alpina-derived crude oils
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Publisher
Database: Elsevier - ScienceDirect
Journal: Food and Bioproducts Processing - Volume 91, Issue 4, October 2013, Pages 617–622
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us