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Ultrasonic pretreatment of corn gluten meal proteins and neutrase: Effect on protein conformation and preparation of ACE (angiotensin converting enzyme) inhibitory peptides

Paper ID Volume ID Publish Year Pages File Format Full-Text
19137 43045 2013 7 PDF Available
Title
Ultrasonic pretreatment of corn gluten meal proteins and neutrase: Effect on protein conformation and preparation of ACE (angiotensin converting enzyme) inhibitory peptides
Abstract

•Ultrasound pretreatment of substrate/enzyme before prepare ACE inhibition peptides.•Neutrase activity increased by 8.74% after sonication at 250 W for 15 min.•ACE inhibition of pretreated at 250 W for 10 min CGM protein increased by 18.27%.•Protein unfolding of CGM and neutrase after sonication was found.

The impact of ultrasound pretreatment of corn gluten meal (CGM) protein and neutrase on the ACE (angiotensin converting enzyme) inhibitory activity of hydrolysate was studied. The UV–vis differential spectra and fluorescence spectra of the CGM protein and neutrase were determined. The neutrase activity and the ACE inhibitory activity of the CGM protein hydrolysates were analyzed. The highest ACE inhibitory activity was obtained at 10 min hydrolysis when the CGM protein was pretreated at 20 kHz for 10–30 min and at a power of 1000 W. The neutrase activity and ACE inhibitory activity were at their maxima at sonication of 250 W and sonication time of 15 min. The fluorescence and UV differential spectra of the proteins of CGM and neutrase reflected molecular unfolding due to sonication. Sonication of the protein substrate and the enzyme before hydrolysis may be an effective way to enhance the ACE inhibition attributes of the hydrolysate.

Keywords
Corn gluten meal; Neutrase; Ultrasound; Protein conformation; ACE inhibitory peptides
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Ultrasonic pretreatment of corn gluten meal proteins and neutrase: Effect on protein conformation and preparation of ACE (angiotensin converting enzyme) inhibitory peptides
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Publisher
Database: Elsevier - ScienceDirect
Journal: Food and Bioproducts Processing - Volume 91, Issue 4, October 2013, Pages 665–671
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us