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A critical evaluation of sampling methods used for assessing microorganisms on surfaces

Paper ID Volume ID Publish Year Pages File Format Full-Text
19145 43046 2010 6 PDF Available
Title
A critical evaluation of sampling methods used for assessing microorganisms on surfaces
Abstract

Methods used to evaluate the effectiveness of cleaning and disinfection regimes, or of putative antimicrobial surfaces, rely on an estimate of the amount of viable microbial cells remaining on a surface after treatment. In essence, microbial cells are applied to the surface, and the number remaining after a specified time/treatment is assessed by variety of methods. This paper provides a critical commentary on these methods.The most common method relies on removal of the cells from the surface, by swabbing or agitation, plating onto culture media, and counting the number of colonies obtained. However, the surface should always be subsequently examined for residual cells: low numbers of colonies are deemed indicative of effective cleaning (i.e. few cells on the surface), but they could also indicate that cells have not been removed from the surface. Swabbing efficiency can be affected by moisture at the surface, presence of organic material, surface topography and presence of antimicrobial compounds.It is important to be aware of the limitations of a given method for assessing the presence of microorganisms on a surface, as well as of the intended antimicrobial property of the surface or agent applied to the surface.

Research highlights▶ Microorganisms not always removed from surface by swabbing. ▶ More than one method recommended for assessing surface contamination. ▶ Cells attached on surfaces should be visualised microscopically, and/or in situ viability assessed.

Keywords
Microbiology; Surface hygiene; Cleaning
First Page Preview
A critical evaluation of sampling methods used for assessing microorganisms on surfaces
Publisher
Database: Elsevier - ScienceDirect
Journal: Food and Bioproducts Processing - Volume 88, Issue 4, December 2010, Pages 335–340
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering