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Evaluation of enzymatic activity of commercial inulinase from Aspergillus niger immobilized in polyurethane foam

Paper ID Volume ID Publish Year Pages File Format Full-Text
19208 43050 2013 6 PDF Available
Title
Evaluation of enzymatic activity of commercial inulinase from Aspergillus niger immobilized in polyurethane foam
Abstract

The objective of this work was to evaluate the stability of the activity of commercial inulinase from Aspergillus niger immobilized in polyurethane foam. The activity of the enzyme was measured in the hydrolysis reaction of both sucrose and inulin at 50 °C and pH 5.5. The enzyme reuse was evaluated during 59 days and 29 reuse cycles. The inulinase immobilized in polyurethane kept 49.7% and 49.4% of its initial activity during 1008 h and 24 reuse cycles for sucrose and inulin, respectively. In this work, enzyme immobilization was accomplished together with the formation of the polyurethane foam, which is an interesting characteristic, differing from several works found in the literature. Taking into account the promising results obtained, the low cost of the immobilization support employed and the whole technique developed, this work may comprise an innovative contribution.

► Enzyme immobilization accomplished together with the formation of the polyurethane foam. ► The low cost of the immobilization support employed. ► Lack found in the literature related to inulinase immobilization in polyurethane foam. ► Enzyme reuse was evaluated during 59 days and 29 reuse cycles.

Keywords
Inulinase; Polyurethane foam; Immobilization
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Evaluation of enzymatic activity of commercial inulinase from Aspergillus niger immobilized in polyurethane foam
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Publisher
Database: Elsevier - ScienceDirect
Journal: Food and Bioproducts Processing - Volume 91, Issue 1, January 2013, Pages 54–59
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us