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A rapid measurement of rutin-degrading enzyme activity in extract of tartary buckwheat seeds

Paper ID Volume ID Publish Year Pages File Format Full-Text
19237 43052 2011 5 PDF Available
Title
A rapid measurement of rutin-degrading enzyme activity in extract of tartary buckwheat seeds
Abstract

In order to measure rutin-degrading enzyme activity conveniently, we have developed an isoabsorptive spectrophotometric method (ISM) for rapidly monitoring rutin-degrading enzyme (RDE) activity in extract of tartary buckwheat seeds. This technique uses discrepancy in absorbency at 372 nm and 344.5 nm, both of which are isoabsorptive wavelengths of rutin, that allow for calculations of quercetin concentrations, which is the only product of the reaction catalyzed by RDE. With this method, extracts containing RDE from buckwheat seeds were analyzed, and the measured data indicated that the sample contained quercetin concentration of 9.909 μg/ml, 8.04 times and 18.08 times greater than negative control (1.232 μg/ml) and positive control (0.548 μg/ml), respectively. ISM results of measuring rutin-degrading enzyme activity in tartary buckwheat seeds correlate with those of HPLC. However, it is beneficial to HPLC as it presents a more convenient and rapid method.

Keywords
HPLC, High Performance Liquid Chromatography; RP-HPLC, Reverse phase High Performance Liquid Chromatography; HSCCC, High-speed Counter-current ChromatographyISM; Rutin; Quercetin; RDE; Isoabsorptive wavelengths; HPLC
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A rapid measurement of rutin-degrading enzyme activity in extract of tartary buckwheat seeds
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Publisher
Database: Elsevier - ScienceDirect
Journal: Food and Bioproducts Processing - Volume 89, Issue 1, January 2011, Pages 81–85
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us