fulltext.study @t Gmail

Influence of nanopatterns on endothelial cell adhesion: Enhanced cell retention under shear stress

Paper ID Volume ID Publish Year Pages File Format Full-Text
1926 95 2009 9 PDF Available
Title
Influence of nanopatterns on endothelial cell adhesion: Enhanced cell retention under shear stress
Abstract

In this study, nanopatterned crosslinked films of collagen Type I were seeded with human microvascular endothelial cells and tested for their suitability for vascular tissue engineering. Since the films will be rolled into tubes with concentric layers of collagen, nutrient transfer through the collagen films is quite crucial. Molecular diffusivity through the collagen films, cell viability, cell proliferation and cell retention following shear stress were studied. Cells were seeded onto linearly nanogrooved films (groove widths of 332.5, 500 and 650 nm), with the grooves aligned in the direction of flow. The nanopatterns did not affect cell proliferation or initial cell alignment; however, they significantly affected cell retention under fluid flow. While cell retention on unpatterned films was 35 ± 10%, it was 75 ± 4% on 332.5 nm patterned films and even higher, 91 ± 5%, on 650 nm patterned films. The films were found to have diffusion coefficients of ca. 10−6 cm2 s−1 for O2 and 4-acetaminophenol, which is comparable to that observed in natural tissues. This constitutes another positive asset of these films for consideration as a scaffold material for vascular tissue engineering.

Keywords
Shear stress; HMEC; Collagen; Nanopattern; Vascular tissue engineering
First Page Preview
Influence of nanopatterns on endothelial cell adhesion: Enhanced cell retention under shear stress
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 5, Issue 7, September 2009, Pages 2451–2459
Authors
, , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us