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Optimization of multi-stage countercurrent extraction of antioxidants from Ginkgo biloba L. leaves

Paper ID Volume ID Publish Year Pages File Format Full-Text
19313 43059 2012 7 PDF Available
Title
Optimization of multi-stage countercurrent extraction of antioxidants from Ginkgo biloba L. leaves
Abstract

Multi-stage countercurrent extraction (MCE) as a novel extraction technique was used to extract antioxidants from Ginkgo biloba leaves. Orthogonal array design (OAD) was employed to optimize the ratio of 60% ethanol to raw material (8–16 mL/g), extraction time (30–60 min) and extraction temperature (60–80 °C) to obtain a high yield of antioxidants from G. biloba leaves by MCE. The optimum conditions were a ratio of 60% ethanol to raw material of 16 mL/g and extraction time of 30 min at 80 °C. Under these conditions, the yields of flavonoids and total phenolics were 1.74% and 2.42%, respectively, and DPPH radicals scavenging activity of the extract was 89.97%. Compared with heat-reflux extraction, MCE had obvious advantages of less extraction time and lower solvent and energy consumption. It may be used as a promising technique for the extraction of bioactive compounds from plant materials.

► HPLC determination of total flavonoids after hydrolysis was established in quality control of Ginkgo biloba leaves extract. ► Multi-stage countercurrent extraction was used to extract antioxidants from Ginkgo biloba leaves. ► Weighted comprehensive evaluation was employed for standardized analysis on three different experimental results.

Keywords
Orthogonal array design; Antioxidant; Flavonoids; Quercetin; Total phenolics
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Optimization of multi-stage countercurrent extraction of antioxidants from Ginkgo biloba L. leaves
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Publisher
Database: Elsevier - ScienceDirect
Journal: Food and Bioproducts Processing - Volume 90, Issue 2, April 2012, Pages 95–101
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us