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Subcritical water extraction of phenolic compounds from pomegranate (Punica granatum L.) seed residues and investigation into their antioxidant activities with HPLC–ABTS+ assay

Paper ID Volume ID Publish Year Pages File Format Full-Text
19328 43059 2012 9 PDF Available
Title
Subcritical water extraction of phenolic compounds from pomegranate (Punica granatum L.) seed residues and investigation into their antioxidant activities with HPLC–ABTS+ assay
Abstract

Subcritical water extraction (SWE) of phenolic compounds from pomegranate seed residues (PSR) was performed. Total phenolic content (TPC) and antioxidant capacities of the extracts obtained at different temperatures were determined, and the individual antioxidant capacities were evaluated by coupling high performance liquid chromatography with ABTS radical reaction system (HPLC–ABTS+). The results showed that the optimum extraction time was 30 min, solid to water ratio was 1:40, and the highest TPC was obtained at 220 °C. The effect of extraction temperature on TPC was significant (p < 0.05), and TPC ranged from 651.7 to 4854.7 (mg/100 g DW) between 100 °C and 220 °C. HPLC–ABTS+ profiles revealed that nine compounds had antioxidant activity. Furthermore, the formation of Maillard reaction products during SWE was investigated, and the content of 5-hydroxymethylfurfural (5-HMF) was determined. The comparative study indicated that SWE was a promising technique for preparation of PSR phenolics.

► Subcritical water extraction of phenolics in pomegranate seed residue was proved to be effective. ► The HPLC-ABTS was an effective analytical technique for the separation of individual phenoilcs. ► Relation between Maillard reaction products and phenolics (by subcritical water) was conducted.

Keywords
Subcritical water extraction; Pomegranate seed residues; Phenolic compounds; Antioxidant activity; HPLC–ABTS+assay
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Subcritical water extraction of phenolic compounds from pomegranate (Punica granatum L.) seed residues and investigation into their antioxidant activities with HPLC–ABTS+ assay
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Publisher
Database: Elsevier - ScienceDirect
Journal: Food and Bioproducts Processing - Volume 90, Issue 2, April 2012, Pages 215–223
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us