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Lactose hydrolysis from milk/whey in batch and continuous processes by concanavalin A-Celite 545 immobilized Aspergillus oryzae β galactosidase

Paper ID Volume ID Publish Year Pages File Format Full-Text
19344 43059 2012 9 PDF Available
Title
Lactose hydrolysis from milk/whey in batch and continuous processes by concanavalin A-Celite 545 immobilized Aspergillus oryzae β galactosidase
Abstract

The present study deals with the immobilization of Aspergillus oryzae β galactosidase on concanavalin A layered Celite 545 as bioaffinity support. The activity yield of crosslinked enzyme was 71%. Michaelis constant, Km was 2.45 mM and 5.58 mM for soluble and crosslinked adsorbed β galactosidase, respectively. Vmax for soluble and crosslinked adsorbed enzyme was 0.52 mM/min and 0.38 mM/min, respectively. Moreover, Kiapp value of crosslinked β galactosidase was 366 × 10−6 M while its soluble counterpart exhibited lower Kiapp value, 181 × 10−6 M at 2% galactose concentration. Soluble and immobilized β galactosidase exhibited same pH and temperature optima at pH 4.5 and 50 °C. The crosslinked adsorbed enzyme retained 90% activity after 1 month of storage at 4 °C and 71% activity after its seventh repeated use. Moreover, crosslinked β galactosidase showed greater resistance to product inhibition mediated by glucose and galactose. Crosslinked Con A-Celite adsorbed β galactosidase showed increased efficiency in hydrolyzing lactose from milk and whey in batch processes at 50 °C as compared to the adsorbed and soluble enzyme. The hydrolysis of lactose in the continuous reactors containing crosslinked β galactosidase was 92% and 81% at flow rate of 20 mL h−1 and 30 mL h−1 after 1 month of operation, respectively.

► The paper summarizes the preparation of a bioaffinity support, concanavalin A layered Celite 545 for the immobilization of Aspergillus oryzae β galactosidase. ► The crosslinked adsorbed β galactosidase exhibited greater stability against various physical and chemical denaturants as compared to the soluble and adsorbed enzyme. ► Moreover, the crosslinked β galactosidase hydrolyzed greater percentage of lactose from milk and whey in batch processes at 50 °C and in continuous reactors at different flow rates.

Keywords
Con A, concanavalin A; ONPG, o-nitrophenyl β-d-galactopyranoside; SβG, soluble β galactosidase; AβG, Con A-Celite 545 adsorbed β galactosidase; CβG, crosslinked Con A-Celite 545 adsorbed β galactosidaseβ Galactosidase; Batch process; Celite 545; Immobiliz
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Lactose hydrolysis from milk/whey in batch and continuous processes by concanavalin A-Celite 545 immobilized Aspergillus oryzae β galactosidase
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Publisher
Database: Elsevier - ScienceDirect
Journal: Food and Bioproducts Processing - Volume 90, Issue 2, April 2012, Pages 351–359
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us