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Recovery of B19 virus-like particles by aqueous two-phase systems

Paper ID Volume ID Publish Year Pages File Format Full-Text
19375 43062 2011 6 PDF Available
Title
Recovery of B19 virus-like particles by aqueous two-phase systems
Abstract

The development of process routes for the recovery of intracellular B19 virus-like particles by aqueous two-phase systems (ATPS) was investigated in this study. Two processes have been developed which may prove advantageous when compared to the conventional recovery processes. One process was designed to recover B19 particles from the bottom phase of a polyethylene glycol (PEG) 1000-magnesium sulphate system while removing cell debris and 31% of total protein with the top phase, interface and sediment. With regard to the analysis of VP1 and VP2 capsid protein after extraction, the yield of B19 particles in the bottom phase was 92.8% or 85.7%, respectively. In an alternative process, B19 particles were recovered from a clarified cell disruptate by interfacial partition. With regard to VP1 and VP2 proteins, 95.3% or 33.2%, respectively was recovered in the interface of a PEG 1000-magnesium sulphate system.

Research highlights▶ The development of process routes for the recovery of intracellular B19 virus-like particles by aqueous two-phase systems (ATPS) was investigated in this study. ▶ Two processes have been developed which may prove advantageous when compared to the conventional recovery processes. ▶ With regard to VP1 and VP2 proteins, 95.3% or 33.2%, respectively was recovered in the interface of a PEG 1000-magnesium sulphate system.

Keywords
Virus-like particles; Aqueous two-phase systems; Conventional recovery processes; Yield; Extraction
First Page Preview
Recovery of B19 virus-like particles by aqueous two-phase systems
Publisher
Database: Elsevier - ScienceDirect
Journal: Food and Bioproducts Processing - Volume 89, Issue 4, October 2011, Pages 322–327
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering