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Effect of kenaf seed oil from different ways of extraction towards ovarian cancer cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
19376 43062 2011 5 PDF Available
Title
Effect of kenaf seed oil from different ways of extraction towards ovarian cancer cells
Abstract

Kenaf (Hibiscus cannabinus) from the family of Malvaceae is a valuable fibre plant native to India and Africa. Kenaf is composed of various active components including tannins, saponins, polyphenolics, alkaloids, essential oils and steroids. It has been used to treat bruises, bilious conditions, fever and puerperium. Nevertheless, the anti-cancer properties of kenaf seed oil have not yet been investigated. In this study, kenaf seed oils obtained by Sonication, Soxhlet and supercritical carbon dioxide fluid extraction (SFE) with 9 different combinations of pressure (bars) and temperature (°C) (200/40, 200/60, 200/80, 400/40, 400/60, 400/80, 600/40, 600/60 and 600/80) were investigated for the cytotoxicities. All the oils were cytotoxic towards ovarian cancer (CaOV3) and colon cancer (HT29) cell lines in a dose dependent manner as detected by using the MTT assay and trypan blue dye exclusion method. Oil from Sonication was the most cytotoxic towards CaOV3 cell line. Treated cells exhibited characteristics of apoptosis such as chromatin condensation and nuclear fragmentation. In conclusion, kenaf seed oils from the three extractions were cytotoxic towards CaOV3 cell line in a dose-dependent manner possibly via the induction of apoptosis. In considering the safety of the product, SFE technology is a better alternative extraction method that is suitable in kenaf seed oil extraction.

Research highlights▶ Supercritical carbon dioxide extraction (SFE) was used to extract kenaf seed oil. ▶ SFE gave high kenaf seed oil yield at pressure of 600 bars. ▶ Kenaf seed oil yield increases with increase in temperature at 600 and 400 bars. ▶ Kenaf seed oil was cytotoxic towards ovarian cancer cells (CaOV3). ▶ Kenaf seed oil induced apoptosis towards ovarian cancer cells (CaOV3).

Keywords
Supercritical carbon dioxide fluid extraction; Kenaf (H. cannabinus) seed oil; Cytotoxicity; Apoptosis
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Effect of kenaf seed oil from different ways of extraction towards ovarian cancer cells
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Publisher
Database: Elsevier - ScienceDirect
Journal: Food and Bioproducts Processing - Volume 89, Issue 4, October 2011, Pages 328–332
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us