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Determination of immobilized enzyme apparent kinetic parameters in packed-bed reactors: Presentation of a new methodology

Paper ID Volume ID Publish Year Pages File Format Full-Text
19441 43065 2008 5 PDF Available
Title
Determination of immobilized enzyme apparent kinetic parameters in packed-bed reactors: Presentation of a new methodology
Abstract

A new explicit equation is proposed for the determination of immobilized enzyme apparent Michaelis–Menten kinetic parameters in packed-bed reactors. The mathematical analysis is based on recycling systems. The present methodology is easily applicable for a wide range of operating conditions. It is free from the probable limitations of the single-pass apparent immobilized enzyme kinetic parameter estimation technique that requires simultaneous measurement of column substrate inlet and effluent concentrations at a specified time. The experimental system is composed of a substrate reservoir and a circulating flow packed-bed immobilized enzyme reactor. The experimental data is gathered for glucose decomposition reaction in a recirculation system with immobilized glucose oxidase on a weak base ion exchanger resin. The apparent kinetic parameter, K′mK′m values were calculated with a new explicit equation, proposed in this work, and they are found to be 0.48 × 10−3 and 0.60 × 10−3 mmol cm−3 for the recirculation rates of 75 and 25 cm3 min−1, respectively.

Keywords
Immobilized enzyme; Kinetics; Modelling; Packed-bed reactor; Recirculation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Food and Bioproducts Processing - Volume 86, Issue 2, June 2008, Pages 104–108
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
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Price after discount Only $4.95
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Full-text PDF Download
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Any Questions? feel free to contact us