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Purification and partial characterization of antifreeze proteins from leaves of Ligustrum lucidum Ait

Paper ID Volume ID Publish Year Pages File Format Full-Text
19451 43066 2011 5 PDF Available
Title
Purification and partial characterization of antifreeze proteins from leaves of Ligustrum lucidum Ait
Abstract

Three AFPs (AFP-1, AFP-2, AFP-3) of Ligustrum lucidum Ait leaves were purified by using ice-affinity, chromatography separation on a DEAE-cellulose-32 column and a Sephadex G100 column. The ice-affinity proteins were about 1.2 mg/100 g leaves. Their molecular weight was 66.1, 26.3, and 20.2 kDa, respectively. Their thermal hysteresis activity was 0.379, 0.678, and 0.460 °C respectively. Asx was very abundant in these AFPs; its molar ratio was 22.2, 24.9, and 27.8%, respectively. AFP-2 was effective to protect peroxidase, β-glucosidase, and trehalose synthase from freeze–thawing process. Cryoprotection of AFP-2 is better than trehalose.

Keywords
Antifreeze protein; Cryoprotection; Purification; Ligustrum lucidum Ait
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Purification and partial characterization of antifreeze proteins from leaves of Ligustrum lucidum Ait
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Publisher
Database: Elsevier - ScienceDirect
Journal: Food and Bioproducts Processing - Volume 89, Issue 2, April 2011, Pages 98–102
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
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Don't Miss Today's Special Offer
Price was $35.95
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Price after discount Only $4.95
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