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The nanocomposite scaffold of poly(lactide-co-glycolide) and hydroxyapatite surface-grafted with l-lactic acid oligomer for bone repair

Paper ID Volume ID Publish Year Pages File Format Full-Text
1950 95 2009 13 PDF Available
Title
The nanocomposite scaffold of poly(lactide-co-glycolide) and hydroxyapatite surface-grafted with l-lactic acid oligomer for bone repair
Abstract

Nanohydroxyapatite (op-HA) surface-modified with l-lactic acid oligomer (LAc oligomer) was prepared by LAc oligomer grafted onto the hydroxyapatite (HA) surface. The nanocomposite of op-HA/PLGA with different op-HA contents of 5, 10, 20 and 40 wt.% in the composite was fabricated into three-dimensional scaffolds by the melt-molding and particulate leaching methods. PLGA and the nanocomposite of HA/PLGA with 10 wt.% of ungrafted hydroxyapatite were used as the controls. The scaffolds were highly porous with evenly distributed and interconnected pore structures, and the porosity was around 90%. Besides the macropores of 100–300 μm created by the leaching of NaCl particles, the micropores (1–50 μm) in the pore walls increased with increasing content of op-HA in the composites of op-HA/PLGA. The op-HA particles could disperse more uniformly than those of pure HA in PLGA matrix. The 20 wt.% op-HA/PLGA sample exhibited the maximum mechanical strength, including bending strength (4.14 MPa) and compressive strength (2.31 MPa). The cell viability and the areas of the attached osteoblasts on the films of 10 wt.% op-HA/PLGA and 20 wt.% op-HA/PLGA were evidently higher than those on the other composites. For the animal test, there was rapid healing in the defects treated with 10 and 20 wt.% op-HA/PLGA, where bridging by a large bony callus was observed at 24 weeks post-surgery. There was non-union of radius defects implanted with PLGA and in the untreated group. This was verified by the Masson’s trichrome staining photomicrographs of histological analysis. All the data extrapolated that the composite with 10 and 20 wt.% op-HA exhibited better comprehensive properties and were the optimal composites for bone repairing.

Keywords
Poly(lactide-co-glycotide); Grafted hydroxyapatite nanoparticles; Mechanical properties; Biocompatibility; Bone repair
First Page Preview
The nanocomposite scaffold of poly(lactide-co-glycolide) and hydroxyapatite surface-grafted with l-lactic acid oligomer for bone repair
Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 5, Issue 7, September 2009, Pages 2680–2692
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering