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A Novel Substrate Addition Method in the 11β-Hydroxylation of Steroids by Curvularia Lunata

Paper ID Volume ID Publish Year Pages File Format Full-Text
19574 43076 2007 10 PDF Available
Title
A Novel Substrate Addition Method in the 11β-Hydroxylation of Steroids by Curvularia Lunata
Abstract

The substrate dissolution in steroids biotransformation is of great importance as it is the bottle-neck for improving the biotransformation ability. In this paper, the effect of organic solvent, surfactant Tween 80, ultrasonic wave and β-cyclodextrins inclusion on the dissolution of substrate RSA during steroids 11β-hydroxylation by Curvularia lunata was studied. Substrate RSA was dissolved in surfactant Tween 80 and then fed to the broth, which notably increased the solubility of substrate in aqueous state, thus improving the hydrocortisone conversion rate. Ultrasonic treatment of substrate or cells both had an obvious effect on the biotransformation. The solubility enhancement of RSA by inclusion complexion with β-cyclodextrins was also evaluated, and the optimum molar ratio of RSA to β-cyclodextrins was 1 : 1. Furthermore the interaction between RSA and β-cyclodextrins in this inclusion complex was assessed by differential scanning calorimetry (DSC) and infrared (IR) spectra. Based on the results of these factors, a novel substrate RSA addition method was performed as follows: β-cyclodextrin was added to RSA blended with 1.2% Tween80 (β-cyclodextrins/RSA 1 : 1, molar ratio), followed by a 20 min sterilization, and then the suspension was fed to the broth. In this way, the hydrocortisone conversion rate was increased by 13.0% than that of the conventional ethanol circumfluence addition method with the RSA addition concentration of 1.0 g L−1.

Keywords
Curvularia lunata; hydrocortisone; solubility; steroid
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A Novel Substrate Addition Method in the 11β-Hydroxylation of Steroids by Curvularia Lunata
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Publisher
Database: Elsevier - ScienceDirect
Journal: Food and Bioproducts Processing - Volume 85, Issue 1, March 2007, Pages 63–72
Authors
, , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us