fulltext.study @t Gmail

Effect of Salts on the Freezing Denaturation of Lactate Dehydrogenase

Paper ID Volume ID Publish Year Pages File Format Full-Text
19617 43084 2006 7 PDF Available
Title
Effect of Salts on the Freezing Denaturation of Lactate Dehydrogenase
Abstract

In this work the effect of added salts in protein solutions on the denaturation of proteins during a freeze–thawing cycle was studied experimentally using lactate dehydrogenase (LDH) as the model proteins. We tested the addition of nine different compounds on their individual effect on LDH during freezing and thawing including inorganic salts, MgSO4, Na2SO4, MgCl2, CaCl2, NaCl, KCl, Mg(NO3)2, Na(NO3) and one organic salt CH3COONa. The effect of freezing and thawing rate on the recovery of LDH activity was experimentally studied using a cryostage set-up. For all the tested solutions, it was observed that slow cooling caused less activity loss of LDH than rapid cooling, confirming our recent results reported elsewhere. Salt type greatly affects the LDH activity recovery, with MgSO4, Na2SO4 and CH3COONa offering improved protection to LDH, and MgCl2, CaCl2 and Mg(NO3)2 causing more damage. The protecting effect of each individual salt roughly follows its position in the so-called Hofmeister Series. The results can guide the selection of protein solution make-up for processes involving freezing preparation, and freezing–thawing protocols.

First Page Preview
Effect of Salts on the Freezing Denaturation of Lactate Dehydrogenase
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Food and Bioproducts Processing - Volume 84, Issue 1, March 2006, Pages 44-50
Authors
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us