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Rational Design Calculation Method for Stepwise Elution Chromatography of Proteins

Paper ID Volume ID Publish Year Pages File Format Full-Text
19620 43084 2006 6 PDF Available
Title
Rational Design Calculation Method for Stepwise Elution Chromatography of Proteins
Abstract

Although linear gradient elution ion-exchange chromatography is best suited for very fine separation of proteins, it is not commonly employed for the actual protein purification process. Instead, stepwise elution is usually used. However, it is difficult to design an efficient stepwise elution process as small changes in the buffer salt concentration often affect the retention volume and the peak width significantly. In this paper, a design calculation method for stepwise elution proposed previously was modified and extended. This method uses the data obtained by linear gradient elution experiments. The efficiency of the process was examined on the basis of the peak width and the separation time. The advantages using convection-aided chromatography media (monolithic columns) were investigated.

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Rational Design Calculation Method for Stepwise Elution Chromatography of Proteins
Publisher
Database: Elsevier - ScienceDirect
Journal: Food and Bioproducts Processing - Volume 84, Issue 1, March 2006, Pages 72-77
Authors
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering