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Surface polyethylene glycol enhances substrate-mediated gene delivery by nonspecifically immobilized complexes

Paper ID Volume ID Publish Year Pages File Format Full-Text
1967 96 2008 14 PDF Available
Title
Surface polyethylene glycol enhances substrate-mediated gene delivery by nonspecifically immobilized complexes
Abstract

Substrate-mediated gene delivery describes the immobilization of gene therapy vectors to a biomaterial, which enhances gene transfer by exposing adhered cells to elevated DNA concentrations within the local microenvironment. Surface chemistry has been shown to affect transfection by nonspecifically immobilized complexes using self-assembled monolayers (SAMs) of alkanethiols on gold. In this report, SAMs were again used to provide a controlled surface to investigate whether the presence of oligo(ethylene glycol) (EG) groups in a SAM could affect complex morphology and enhance transfection. EG groups were included at percentages that did not affect cell adhesion. Nonspecific complex immobilization to SAMs containing combinations of EG- and carboxylic acid-terminated alkanethiols resulted in substantially greater transfection than surfaces containing no EG groups or SAMs composed of EG groups combined with other functional groups. Enhancement in transfection levels could not be attributed to complex binding densities or release profiles. Atomic force microscopy imaging of immobilized complexes revealed that EG groups within SAMs affected complex size and appearance and could indicate the ability of these surfaces to preserve complex morphology upon binding. The ability to control the morphology of the immobilized complexes and influence transfection levels through surface chemistry could be translated to scaffolds for gene delivery in tissue engineering and diagnostic applications.

Keywords
Gene delivery; Reverse transfection; Atomic force microscopy (AFM); Self-assembled monolayers
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Surface polyethylene glycol enhances substrate-mediated gene delivery by nonspecifically immobilized complexes
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Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 4, Issue 1, January 2008, Pages 26–39
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us