fulltext.study @t Gmail

Multiplex PCR for the early detection of fumonisin producing Fusarium verticillioides

Paper ID Volume ID Publish Year Pages File Format Full-Text
19671 43116 2016 5 PDF Available
Title
Multiplex PCR for the early detection of fumonisin producing Fusarium verticillioides
Abstract

In the present study, fumonisin producing Fusarium verticillioides was specifically detected in pure cultures, cereal samples and plant materials by multiplex PCR using one forward VERTF-1 and two reverse primers VERTR and VERTF-2. A total of 326 Fusarium isolates were obtained from maize, sorghum, paddy wheat and pearl millet samples collected from different districts of Karnataka, India. All Fusarium species were subjected to single round of PCR with species specific and fumonisin specific primers which recorded 59.50% of F. verticillioides and 53.98% of fumonisin producing F. verticillioides. Maize samples recorded highest frequency 34.42% of fumonisin producing F. verticillioides followed by paddy 28.57% and sorghum 16.66%. Sensitivity of multiplex PCR experiment was conducted by whole grain experiment of the collected cereals, roots and leaves of the cereal samples by diluting the DNA 10 to 100 times in which 1:50, 1:75 and 1:100 diluted samples recorded positive. The developed multiplex PCR assay provided a powerful tool for the accurate detection, identification and discrimination of potential fumonisin producing F. verticillioides strains among the population. The present study is the first report of developing the multiplex PCR method for early detection of fumonisin producing F. verticillioides from cereal samples, pure cultures and plant parts.

Keywords
Fusarium; Cereals; Maize; Paddy; Sorghum; Spiking; Mycotoxin
First Page Preview
Multiplex PCR for the early detection of fumonisin producing Fusarium verticillioides
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Food Bioscience - Volume 13, 1 March 2016, Pages 84–88
Authors
, , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us