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Immobilization of cellobiose 2-epimerase from Caldicellulosiruptor saccharolyticus on commercial resin Duolite A568

Paper ID Volume ID Publish Year Pages File Format Full-Text
19678 43117 2016 7 PDF Available
Title
Immobilization of cellobiose 2-epimerase from Caldicellulosiruptor saccharolyticus on commercial resin Duolite A568
Abstract

•After thermal-purification, CsCE was immobilized on Duolite A568 through ionic attraction and glutaraldehyde crosslinking.•Crude enzyme after thermal-treatment at 70 °C for 2 h was purified by 6.14-folds and used directly for immobilization.•Enzyme adsorption on resin was carried out at pH 6.5, 35 °C for 1 h and over 70% of the enzyme was adsorbed.•Immobilized CsCE exhibited excellent thermal and recycling stability.•Immobilized CsCE presented higher activity at 40–70 °C and enabling it to produce lactulose at lower temperature.

This work presented a comprehensive method to immobilize cellobiose 2-epimerase from Caldicellulosiruptor saccharolyticus (CsCE) on a commercial resin Duolite A568. After thermal-treatment at 70 °C for 2 h, crude enzyme was purified by 6.14-fold with enzyme recovery of 86.6%. The obtained thermal-purified enzyme then adsorbed on Duolite A568 through electrostatic attractions at pH 6.5, 35 °C for 1 h and over 70% of the enzyme was adsorbed. The adsorbed enzyme subsequently stabilized with 0.3% (v/v) glutaraldehyde at 4 °C for 1.5 h. The immobilized enzyme was stable in pH range from 6 to 9 and can be easily desorbed at acidic (<5) or basic pH (>10). As compared to its free form, immobilized CsCE was more active at middle temperatures (40–80 °C) and maintained almost 100% activity at 50 °C for over 12 h. In addition, lactulose production carried out by immobilized CsCE at 70 °C was almost equal to that obtained by free CsCE at 80 °C. The results obtained were very encouraging and supports the feasibility of lactulose bioconversion by immobilized CsCE on an industrial scale.

Keywords
Cellobiose 2-epimerase; Duolite A568; Immobilized enzyme; Enzymatic properties; Lactulose production
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Immobilization of cellobiose 2-epimerase from Caldicellulosiruptor saccharolyticus on commercial resin Duolite A568
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Publisher
Database: Elsevier - ScienceDirect
Journal: Food Bioscience - Volume 14, 1 June 2016, Pages 47–53
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us