fulltext.study @t Gmail

A fibrin degrading serine metallo protease of Bacillus circulans with α-chain specificity

Paper ID Volume ID Publish Year Pages File Format Full-Text
19689 43118 2015 7 PDF Available
Title
A fibrin degrading serine metallo protease of Bacillus circulans with α-chain specificity
Abstract

The addition of a fibrinolytic enzyme from Bacillus to a food system contributes to its functionality and may help in preventive prophylaxis of thrombosis-related disorders. In the present study, a fibrinolytic serine-metallo protease with substrate specificity was identified and partially purified from Bacillus circulans CFR11. The enzyme was active toward α-chain moiety of human fibrin and Aα and Bβ chains of fibrinogen and was termed as CFR11-protease. With the specific activity of 1399.57 U/mg of protein, maximum activity of enzyme was observed at pH 7.4 and temperature of 50 °C. Divalent cations Mg2+ and Mn2+ were found to enhance protease activity but it was inhibited by phenyle methyl sulphonyl fluoride (PMSF) and ethylene diamine tetra acetic acid (EDTA). From the data obtained, the enzyme had an apparent molecular weight of 190 kDa as per SDS-PAGE analysis and zymography. The in-vitro Activated Partial Thromboplastin Time (APTT) and Prothrombin Time (PT) values showed no significant difference in both dose and time-dependent assay, inferring the safer property of the CFR11 enzyme for thrombolytic purpose. From these results it can be concluded that a new enzyme CFR11 protease, isolated with specificity towards α-chain moiety of fibrin, can become an effective fibrin specific agent for the safe prophylaxis of thrombosis.

Keywords
Bacillus circulans; Fibrino(geno)lytic activity; α-Chain; Serine-metallo protease; Zymogram; Thrombolytic
First Page Preview
A fibrin degrading serine metallo protease of Bacillus circulans with α-chain specificity
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Food Bioscience - Volume 11, 1 September 2015, Pages 72–78
Authors
, ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us