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BOX-PCR and PCR-DGGE analysis for bacterial diversity of a naturally fermented functional food (Enzyme®)

Paper ID Volume ID Publish Year Pages File Format Full-Text
19712 43120 2014 8 PDF Available
Title
BOX-PCR and PCR-DGGE analysis for bacterial diversity of a naturally fermented functional food (Enzyme®)
Abstract

Enzyme® product is a popular macrobiotic food in Asia, which is naturally fermented using vegetables, fruits, plant leaves, and flour as raw materials. To study the bacterial diversity in a commercial available Enzyme® product, culture-dependent BOX-PCR analysis and culture-independent PCR-DGGE analysis were used simultaneously. The amount of the cultivable bacteria in the product is about 2.5×107 CFU/g. A total of 329 isolated strains were grouped into six fingerprint types according to BOX-PCR analysis. Four dominant species were identified among the six groups, which are Bacillus coagulans (60.2%), Lactobacillus plantarum (23.7%), Lactobacillus oris (8.2%), and Staphylococcus epidermidis (7.9%). Other five unculturable species, which were not cultivated by the condition used in this study, were identified according to PCR-DGGE analysis. Among them, Phyllobacterium myrsinacearum, Rhodococcus erythropolis, and Acidovorax konjaci were frequently found to be associated with plant materials. Therefore, combinatory application of culture-dependent and -independent techniques allowed us to unravel the microbial diversity in a naturally fermented health-promoting food product.

Keywords
Naturally fermented food; Functional food; BOX-PCR; Denaturing gradient gel electrophoresis (DGGE)
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BOX-PCR and PCR-DGGE analysis for bacterial diversity of a naturally fermented functional food (Enzyme®)
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Publisher
Database: Elsevier - ScienceDirect
Journal: Food Bioscience - Volume 5, March 2014, Pages 115–122
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us