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Stimulatory effects of genistein and quercetin on the proliferation of MCF-7 cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
19764 43126 2013 9 PDF Available
Title
Stimulatory effects of genistein and quercetin on the proliferation of MCF-7 cells
Abstract

•Gen and Que increased cell viability in MCF-7 cells, either with or without E2.•Gen and Que with E2 induced inhibition of cell apoptosis.•Gen and Que with E2 promoted cell distribution to G2 and S phases.•ER and PI3K/Akt pathways were activated after treatment cells with Gen and Que.•The activation of Akt may occur downstream of the ERα activation.

Flavonoids are plant-derived compounds which exert estrogenic activities and have potential to interfere cell growth in estrogen-dependent cells. In this study, we investigated the effects of two widespread flavonoids, genistein and quercetin, on cell proliferation in MCF-7 human breast cancer cells. We found that both flavonoids significantly increased cell viability, either in presence or in absence of 17β-estradiol (E2). The proliferation effect was noticeably reduced by fulvestrant, an antiestrogen, and by LY294002, an inhibitor of phosphatidylinositol 3 kinase (PI3K)/Akt pathway. Western blot analysis confirmed that estrogen receptor (ER) and PI3K/Akt pathways were activated after treatment with these two flavonoids. The results also suggested that the activation of Akt may occur downstream of the ERα activation. The present study might open new perspectives of the action of flavonoids, and suggested that flavonoids could stimulate cell proliferation and trigger estrogenic responses, in part mediated by ER and PI3K/Akt pathways in MCF-7 cells.

Keywords
Flavonoids; MCF-7 cells; Estrogen receptor; PI3K/Akt; Cell proliferation
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Stimulatory effects of genistein and quercetin on the proliferation of MCF-7 cells
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Publisher
Database: Elsevier - ScienceDirect
Journal: Food Bioscience - Volume 2, June 2013, Pages 15–23
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
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Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
Online Support
Any Questions? feel free to contact us