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Polyphenolic extract of Blighia sapida arilli prevents N-nitrosodiethylamine-mediated oxidative onslaught on microsomal protein, lipid and DNA

Paper ID Volume ID Publish Year Pages File Format Full-Text
19782 43128 2013 9 PDF Available
Title
Polyphenolic extract of Blighia sapida arilli prevents N-nitrosodiethylamine-mediated oxidative onslaught on microsomal protein, lipid and DNA
Abstract

•Blighia sapida scavenges reactive oxygen species.•Blighia sapida prevents NDEA-induced oxidative stress in rat microsomes.•Blighia sapida prevents NDEA-induced oxidation of microsomal protein.•Blighia sapida prevents NDEA-induced peroxidation of microsomal lipids.•Blighia sapida prevents NDEA-induced fragmentation of microsomal DNA.

Polyphenolic extract of Blighia sapida arilli was evaluated for reactive oxygen species (ROS) scavenging and detoxification potentials in rat microsomes. ROS scavenging potentials of the polyphenolic extract of B. sapida (0.2–1.0 mg/mL) was investigated using DPPH radical, superoxide anion radical, hydrogen peroxide, hydroxyl radical and ferric ion reducing system. The detoxification of ROS was evaluated in N-nitrosodiethylamine-induced redox imbalance in rat microsomes. B. sapida polyphenolic extract (1.0 mg/mL) scavenged DPPH, superoxide anion radical, hydrogen peroxide, and hydroxyl radical by 60, 67, 63, and 57%, respectively, while ferric ion was significantly reduced. N-nitrosodiethylamine-mediated decrease in the activities of ROS detoxifying enzymes was significantly (P<0.05) attenuated. N-nitrosodiethylamine-mediated elevation in the concentrations of oxidative stress biomarkers: malondialdehyde, conjugated dienes, lipid hydroperoxides, protein carbonyl and percentage DNA fragmentation were significantly (P<0.05) lowered by B. sapida polyphenolic extract. Overall, the polyphenolic extract of B. sapida arilli elicited ROS scavenging and detoxification potentials and prevented lipid peroxidation, protein oxidation and DNA fragmentation.

Keywords
Blighia sapida; N-nitrosodiethylamine; Reactive oxygen species; Protein carbonyl; DNA fragmentation
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Polyphenolic extract of Blighia sapida arilli prevents N-nitrosodiethylamine-mediated oxidative onslaught on microsomal protein, lipid and DNA
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Publisher
Database: Elsevier - ScienceDirect
Journal: Food Bioscience - Volume 1, March 2013, Pages 48–56
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us