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Structure and antioxidant activity of whey protein isolate conjugated with glucose via the Maillard reaction under dry-heating conditions

Paper ID Volume ID Publish Year Pages File Format Full-Text
19942 43148 2014 10 PDF Available
Title
Structure and antioxidant activity of whey protein isolate conjugated with glucose via the Maillard reaction under dry-heating conditions
Abstract

The aim of this study was to investigate the structural characteristics and antioxidant activities of the Maillard reaction products obtained from whey protein isolate (WPI) and glucose (1:1 weight ratio) after dry-heating (60 °C and 79% relative humidity) for 0–7 d. The results revealed that the color (redness and yellowness), the UV–vis absorption, and the fluorescence intensity increased as the reaction time increased (P < 0.05). In addition, marked increases in the reducing power and the ABTS radical scavenging activities of the WPI–glucose conjugates were obtained with an increase in the reaction time (P < 0.05). The sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis illustrated that WPI and glucose formed high molecular weight conjugates. The increased Tmax demonstrated that the thermal stability of WPI was remarkably improved by its conjugation with glucose. The Fourier transform infrared (FT-IR) spectra suggested that the amide I, II, and III bands of WPI were altered by the Maillard reaction. All in all, these results suggested that WPI–glucose conjugates have high thermal stability and improved antioxidant properties.

Keywords
Whey protein isolate; Glucose; Conjugation; Maillard reaction; Structure; Antioxidant activity
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Structure and antioxidant activity of whey protein isolate conjugated with glucose via the Maillard reaction under dry-heating conditions
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Publisher
Database: Elsevier - ScienceDirect
Journal: Food Structure - Volume 1, Issue 2, April 2014, Pages 145–154
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us