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Recovery of laccase from processed Hericium erinaceus (Bull.:Fr) Pers. fruiting bodies in aqueous two-phase system

Paper ID Volume ID Publish Year Pages File Format Full-Text
19959 43150 2016 6 PDF Available
Title
Recovery of laccase from processed Hericium erinaceus (Bull.:Fr) Pers. fruiting bodies in aqueous two-phase system
Abstract

•Laccase was successful recovered from processed H. erinaceus in PEG/Salt ATPS.•Cold-stored of H. erinaceus recorded highest laccase activities (2.02 ± 0.04 U/mL).•Recovery conditions of laccase in optimized system of APTS contained 17% (w/w) PEG-8000 and 12.2% (w/w) potassium phosphate.

The feasible use of aqueous two-phase systems (ATPSs) to establish a viable protocol for the recovery of laccase from processed Hericium erinaceus (Bull.:Fr.) Pers. fruiting bodies was evaluated. Cold-stored (4.00 ± 1.00°C) H. erinaceus recorded the highest laccase activities of 2.02 ± 0.04 U/mL among all the processed techniques. The evaluation was carried out in twenty-five ATPSs, which composed of polyethylene glycol (PEG) with various molecular weights and potassium phosphate salt solution to purify the protein from H. erinaceus. Optimum recovery condition was observed in the ATPS which contained 17% (w/w) PEG with a molecular weight of 8000 and 12.2% (w/w) potassium phosphate solution, at a volume ratio (VR) of 1.0. The use of ATPS resulted in one-single primary recovery stage process that produced an overall yield of 99% with a purification factor of 8.03 ± 0.46. The molecular mass of laccases purified from the bottom phase was in the range of 55–66 kDa. The purity of the partitioned laccase was confirmed with sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE).

Keywords
Aqueous two-phase; Protein recovery; Purification; Separation; Hericium erinaceus; Laccase
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Recovery of laccase from processed Hericium erinaceus (Bull.:Fr) Pers. fruiting bodies in aqueous two-phase system
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 122, Issue 3, September 2016, Pages 301–306
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us