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Genome-wide gene expression analysis of mouse embryonic stem cells exposed to p-dichlorobenzene

Paper ID Volume ID Publish Year Pages File Format Full-Text
19963 43150 2016 5 PDF Available
Title
Genome-wide gene expression analysis of mouse embryonic stem cells exposed to p-dichlorobenzene
Abstract

•We exposed mESCs to p-dichlorobenzene 1 or 28 days (daily dose).•Mechanisms modulating proteins occurred following acute and chronic exposures.•LncRNAs are novel biomarkers of the chemical stress response in mESCs.

Because of the limitations of whole animal testing approaches for toxicological assessment, new cell-based assay systems have been widely studied. In this study, we focused on two biological products for toxicological assessment: mouse embryonic stem cells (mESCs) and long noncoding RNAs (lncRNAs). mESCs possess the abilities of self-renewal and differentiation into multiple cell types. LlncRNAs are an important class of pervasive non-protein-coding transcripts involved in the molecular mechanisms associated with responses to chemicals. We exposed mESCs to p-dichlorobenzene (p-DCB) for 1 or 28 days (daily dose), extracted total RNA, and performed deep sequencing analyses. The genome-wide gene expression analysis indicated that mechanisms modulating proteins occurred following acute and chronic exposures, and mechanisms modulating genomic DNA occurred following chronic exposure. Moreover, our results indicate that three novel lncRNAs (Snora41, Gm19947, and Scarna3a) in mESCs respond to p-DCB exposure. We propose that these lncRNAs have the potential to be surrogate indicators of p-DCB responses in mESCs.

Keywords
Non-coding RNA; Mouse embryonic stem cells; Deep sequencing; p-Dichlorobenzene; Toxicological assessment
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Genome-wide gene expression analysis of mouse embryonic stem cells exposed to p-dichlorobenzene
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 122, Issue 3, September 2016, Pages 329–333
Authors
, , , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us