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In vitro steroid profiling system for the evaluation of endocrine disruptors

Paper ID Volume ID Publish Year Pages File Format Full-Text
19970 43150 2016 8 PDF Available
Title
In vitro steroid profiling system for the evaluation of endocrine disruptors
Abstract

Endocrine disruptors (ED) are chemicals that affect various aspects of the endocrine system, often leading to the inhibition of steroidogenesis. Current chemical safety policies that restrict human exposure to such chemicals describe often time-consuming and costly methods for the evaluation of ED effects. We aimed to develop an effective tool for accurate phenotypic chemical toxicology studies. We developed an in vitro ED evaluation system using gas chromatography/mass spectrometry (GC/MS/MS) methods for metabolomic analysis of multi-marker profiles. Accounting for sample preparation and GC/MS/MS conditions, we established a screening method that allowed the simultaneous analysis of 17 steroids with good reproducibility and a linear calibration curve. Moreover, we applied the developed system to H295R human adrenocortical cells exposed to forskolin and prochloraz in accordance with the Organization for Economic Cooperation and Development (OECD) guidelines and observed dose-dependent variations in steroid profiles. While the OECD guidelines include only testosterone and 17β-estradiol, our system enabled a comprehensive and highly sensitive analysis of steroid profile alteration due to ED exposure. The application of our ED evaluation screen could be economical and provide novel insights into the hazards of ED exposure to the endocrine system.

Keywords
Endocrine disruptors; Steroid; Metabolic profiling; H295R cells; Gas chromatography/mass spectrometry
First Page Preview
In vitro steroid profiling system for the evaluation of endocrine disruptors
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 122, Issue 3, September 2016, Pages 370–377
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering