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High acetone–butanol–ethanol production in pH-stat co-feeding of acetate and glucose

Paper ID Volume ID Publish Year Pages File Format Full-Text
19997 43152 2016 7 PDF Available
Title
High acetone–butanol–ethanol production in pH-stat co-feeding of acetate and glucose
Abstract

We previously reported the metabolic analysis of butanol and acetone production from exogenous acetate by 13C tracer experiments (Gao et al., RSC Adv., 5, 8486–8495, 2015). To clarify the influence of acetate on acetone–butanol–ethanol (ABE) production, we first performed an enzyme assay in Clostridium saccharoperbutylacetonicum N1-4. Acetate addition was found to drastically increase the activities of key enzymes involved in the acetate uptake (phosphate acetyltransferase and CoA transferase), acetone formation (acetoacetate decarboxylase), and butanol formation (butanol dehydrogenase) pathways. Subsequently, supplementation of acetate during acidogenesis and early solventogenesis resulted in a significant increase in ABE production. To establish an efficient ABE production system using acetate as a co-substrate, several shot strategies were investigated in batch culture. Batch cultures with two substrate shots without pH control produced 14.20 g/L butanol and 23.27 g/L ABE with a maximum specific butanol production rate of 0.26 g/(g h). Furthermore, pH-controlled (at pH 5.5) batch cultures with two substrate shots resulted in not only improved acetate consumption but also a further increase in ABE production. Finally, we obtained 15.13 g/L butanol and 24.37 g/L ABE at the high specific butanol production rate of 0.34 g/(g h) using pH-stat co-feeding method. Thus, in this study, we established a high ABE production system using glucose and acetate as co-substrates in a pH-stat co-feeding system with C. saccharoperbutylacetonicum N1-4.

Keywords
Acetone–butanol–ethanol production; Acetate; pH-stat; Enzymatic analysis; Clostridium saccharoperbutylacetonicum
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High acetone–butanol–ethanol production in pH-stat co-feeding of acetate and glucose
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 122, Issue 2, August 2016, Pages 176–182
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us