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Characterization of a halotolerant acetoclastic methanogen highly enriched from marine sediment and its application in removal of acetate

Paper ID Volume ID Publish Year Pages File Format Full-Text
20020 43153 2016 7 PDF Available
Title
Characterization of a halotolerant acetoclastic methanogen highly enriched from marine sediment and its application in removal of acetate
Abstract

A marine sediment collected from Hiroshima Bay was cultured in artificial seawater, containing 0.51 M NaCl and 60 mM acetate and was found to exhibit active methane production at 37°C. Following four successive serial dilutions of cultures in medium containing 0.51 M NaCl, 60 mM acetate, and antibiotics, the well-acclimated methanogen was found to exhibit growth over a range of NaCl concentration (between 0 M and 2.06 M). The specific growth rates of the highly enriched methanogen, termed strain HA, in the absence of NaCl and in the presence of 1.54 M NaCl were estimated to be 0.037 h−1 and 0.027 h−1, respectively. The pH and temperature for optimum growth were determined to be 7.0–8.8 and 37°C, respectively. Although cells that had morphology similar to Methanosaeta sp. became dominant in the culture, methane production was still detected in the medium containing 0.51 M NaCl and other substrates such as methanol, formate, and methylamine, indicating contamination with other methanogens. The phylogenetic tree based on 16S rRNA gene sequences revealed that the strain HA was closely related to Methanosaeta harundinacea 6Ac and 8AcT, with sequence similarity of 98% and 97%, respectively. The continuous removal of acetate with upflow anaerobic filter reactor for industrial use of strain HA determined a methane production rate of 70 mM/d under condition of 0.51 M NaCl and successful methane production even under 1.54 M NaCl.

Keywords
Halotolerant; Acetoclastic methanogen; Marine sediment; Methanosaeta; Methane fermentation; Continuous culture; Upflow anaerobic filter reactor
First Page Preview
Characterization of a halotolerant acetoclastic methanogen highly enriched from marine sediment and its application in removal of acetate
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 121, Issue 2, February 2016, Pages 196–202
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering