fulltext.study @t Gmail

Characteristics of human cell line, F2N78, for the production of recombinant antibody in fed-batch and perfusion cultures

Paper ID Volume ID Publish Year Pages File Format Full-Text
20040 43154 2016 8 PDF Available
Title
Characteristics of human cell line, F2N78, for the production of recombinant antibody in fed-batch and perfusion cultures
Abstract

A human hybrid cell line, F2N78, was developed by somatic fusion of HEK293 and Namalwa cells for the production recombinant biopharmaceutical proteins. In this study, we performed perfusion culture to verify its potential in culture process used for human cell expression platform. Cell viability could be maintained over 90% and high viable cell density was obtained at higher than 1.0 × 107 cells/mL by bleeding process in perfusion culture. The cells were adapted well in both culture modes, but there were apparent differences in protein quality. Compared to fed-batch culture, degalactosylated forms such as G0F and G0 as well as Man5 showed no significant increases in perfusion culture. In terms of charge variants, acidic peaks increased, whereas main peaks constantly decreased according to the length of culture period in both methods.

Keywords
Human cells; F2N78 cells; Fed-batch culture; Perfusion culture; Glycosylation
First Page Preview
Characteristics of human cell line, F2N78, for the production of recombinant antibody in fed-batch and perfusion cultures
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 121, Issue 3, March 2016, Pages 317–324
Authors
, , , , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering