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Extra-facile chiral separation of amino acid enantiomers by LC-TOFMS analysis

Paper ID Volume ID Publish Year Pages File Format Full-Text
20045 43154 2016 5 PDF Available
Title
Extra-facile chiral separation of amino acid enantiomers by LC-TOFMS analysis
Abstract

An extra-facile chiral liquid chromatography-time of flight mass spectrometry (LC-TOFMS) analytical method of amino acid enantiomers has been developed without a derivatization process. The enantioseparation of eighteen proteinogenic amino acids (except for proline) was simultaneously performed using a combination of a chiral column (CROWNPAK CR-I(+)) and a TOFMS within 15 min. An isocratic condition of a simple mobile phase comprising acetonitrile/water/trifluoroacetic acid (96/4/0.5) gave baseline separation of all underivatized amino acid enantiomers on the chiral column.

Keywords
d-Amino acids; Underivatized amino acids; Chiral liquid chromatography; Time of flight mass spectrometry
First Page Preview
Extra-facile chiral separation of amino acid enantiomers by LC-TOFMS analysis
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 121, Issue 3, March 2016, Pages 349–353
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering