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Direct recovery of cyclodextringlycosyltransferase from Bacillus cereus using aqueous two-phase flotation

Paper ID Volume ID Publish Year Pages File Format Full-Text
20058 43155 2015 6 PDF Available
Title
Direct recovery of cyclodextringlycosyltransferase from Bacillus cereus using aqueous two-phase flotation
Abstract

Purification of cyclodextrin glycosyl transferase (CGTase) from Bacillus cereus using polyethylene glycol (PEG)-potassium phosphates aqueous two-phase flotation (ATPF) system was studied in this paper. The effects of varying PEG molecular weight, tie-line length (TLL) value, volume ratio (VR), pH value, crude concentration and gas nitrogen flotation time were investigated. The optimal condition for purification of CGTase was attained at 18.0% (w/w) PEG 8000, 7.0% (w/w) potassium phosphates, VR of 3.0, 20% (w/w) crude load at pH 7, and 80 min nitrogen flotation time at a flow rate of 5 L/min. With this optimal condition, purification factor (PFT) of 21.8 and a yield (YT) of 97.1% were attained. CGTase was successfully purified in a single downstream processing step using the ATPF.

Keywords
Cyclodextringlycosyltransferase; Aqueous two-phase flotation; Bacillus cereus; Purification; Polyethylene glycol-salt system
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Direct recovery of cyclodextringlycosyltransferase from Bacillus cereus using aqueous two-phase flotation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 120, Issue 6, December 2015, Pages 684–689
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us